flow cytometer stratedigm s1000  (Stratedigm Inc)

Journal: eLife

Article Title: A concerted mechanism involving ACAT and SREBPs by which oxysterols deplete accessible cholesterol to restrict microbial infection

doi: 10.7554/eLife.83534

Figure Lengend Snippet:

Article Snippet: One aliquot (200 µl) was transferred to a tube containing 5 µl of a stock solution of fALOD4-Neon (final concentration of 1 µM) and incubated at RT for 30 min. A portion of this mixture (150 µl) was then transferred to a 96-well plate and fALOD4-Neon binding was measured by analyzing 10,000 RBCs for each condition on a Stratedigm S1000 EX Flow Cytometer (BD Biosciences).

Techniques: Transfection, Construct, Flow Cytometry, Recombinant, Sequencing, Bicinchoninic Acid Protein Assay, Microscopy, Protease Inhibitor, Modification, Methylation, Software, CRISPR, Imaging